| Name | Title | Country | Period |
| Peter Gerard | Abbott Master | French | 1087-1120 |
| Raymond de Puy (Pius) | Master | French | 1120-1158/60 |
| Auger de Balben | Master | French | 1158/60-1162/3 |
| Arnaud de Comps | Master | French | 1162-1163 |
| Gilbert d'Assailly | Master | English | 1163-1169/70 |
| Gaston de Murols | Master | | 1170-1172 |
| Joubert of Syria | Master | | 1172-1177 |
| Roger de Moulins | Master | | 1177-1187 |
| Ermengard d’Asp | Master | | 1188-1190 |
| Garnier de Naplous | Master | English | 1189/90-1192 |
| Geoffrey de Donjon | Master | French | 1192/3-1202 |
| Alphonse de Portugal | Master | Portuguese | 1202-1206 |
| Geoffroy le Rat | Master | French | 1206-1207 |
| Garin de Montaigu | Master | French | 1207-1227/8 |
| Bertrand de Thessy | Master | French | 1228-1231 |
| Guerin Lebrun | Master | | 1231-1236 |
| Bertrand de Comps | Master | French | 1236-1239/40 |
| Pierre de Vieille-Brioude | Master | | 1239/40-1242 |
| Guillaume de Chateauneuf | Master | French | 1242-1258 |
| Hugues de Revel | Grand Master | French | 1258-1277 |
| Nicolas Lorgne | Grand Master | | 1277/8-1284 |
| Jean de Villiers | Grand Master | French | 1285-1293/4 |
| Odon de Pins | Grand Master | French | 1294-1296 |
| Guillaume de Villaret | Grand Master | French | 1296-1305 |
| Foulques de Villaret | Grand Master | French | 1305-1319 |
| Helion de Villeneuve | Grand Master | French | 1319-1346 |
| Dieudonne de Gozon | Grand Master | French | 1346-1353 |
| Pierre de Corneillan | Grand Master | French | 1353-1355 |
| Roger de Pins | Grand Master | French | 1355-1365 |
| Raymond Berenger | Grand Master | French | 1365-1374 |
| Robert de Juilliac | Grand Master | French | 1374-1377 |
| Jean Fernandez de Heredia | Grand Master | Spanish | 1377-1383 |
| Ricardo Caracciolo | Grand Master | Italian | 1383-1395 |
| Philibert de Naillac | Grand Master | French | 1396-1421 |
| Antoine Fluvian de la Riviere | Grand Master | Spanish | 1421-1437 |
| Jean de Lastic | Grand Master | French | 1437-1454 |
| Jacques de Milly | Grand Master | French | 1454-1461 |
| Pierre Raymond Zacosta | Grand Master | Spanish | 1461-1467 |
| Jean-Baptiste Orsini | Grand Master | Italian | 1467-1476 |
| Pierre d'Aubusson, Cardinal | Grand Master | French | 1476-1503 |
| Emery d'Amboise dit Chaumont | Grand Master | French | 1503-1512 |
| Guy de Blanchefort | Grand Master | French | 1512-1513 |
| Fabrice del Carretto | Grand Master | Italian | 1513-1521 |
| Philippe Villiers de l’Isle-Adam | Grand Master | French | 1521-1534 |
| Pierre del Ponte | Grand Master | Italian | 1534-1535 |
| Didier de Saint-Jaille | Grand Master | French | 1535-1536 |
| Jean de Homedes y Coscon | Grand Master | Aragonese | 1536-1553 |
| Claude de la Sengle | Grand Master | French | 1553-1557 |
| Jean Parisot de Valette | Grand Master | French | 1557-1568 |
| Pietro Ciocchi del Monte San Savino | Grand Master | Italian | 1568-1572 |
| Jean l'Evesque de la Cassière | Grand Master | French | 1572-1582 |
| Hugues Loubenx de Verdale, Cardinal | Grand Master | French | 1582-1595 |
| Martin Garzes | Grand Master | Aragonese | 1595-1601 |
| Alof de Wignacourt | Grand Master | French | 1601-1622 |
| Luis Mendes de Vasconcellos | Grand Master | Portuguese | 1622-1623 |
| Antoine de Paule | Grand Master | French | 1623-1636 |
| Jean-Baptiste Lascaris de Castellar | Grand Master | French | 1636-1657 |
| Martin de Redin y Cruzat | Grand Master | Aragonese | 1657-1660 |
| Annet de Clermont de Chattes Gessan | Grand Master | French | 1660 |
| Rafael Cotoner y de Oleza | Grand Master | Aragonese | 1660-1663 |
| Nicholas Cotoner y de Oleza | Grand Master | Aragonese | 1663-1680 |
| Gregorio Carafa della Roccella | Grand Master | Italian | 1680-1690 |
| Adrien de Wignacourt | Grand Master | French | 1690-1697 |
| Ramon Perellos y Rocafull | Grand Master | Aragonese | 1697-1720 |
| Marcantonio Zondadari | Grand Master | Italian |
1720-1722 |
| Antonio Manoel de Vilhena | Grand Master | Portuguese | 1722-1736 |
| Ramon Despuig y Martinez de Marcilla | Grand Master | Aragonese | 1736-1741 |
| Manuel Pinto de Fonseca | Grand Master | Portuguese | 1741-1773 |
| Francisco Ximenez de Texada | Grand Master | Aragonese | 1773-1775 |
| Emmanuel de Rohan de Polduc | Grand Master | French | 1775-1797 |
| Ferdinand Von Hompesch zu Bolheim | Grand Master | German | 1797-1798 |
| Paul I, Emperor of Russia | Grand Master | Russian | 1798-1801 |
| Grand Duke Alexander of Russia | Grand Master | Russian | 1913-1933 |
| Crolian Edelen de Burgh | Grand Master | American | 1960-1973 |
| Prince Roberto Paterno | Grand Master | Italian | 1974-1993 |
| Prince George Korey-Krzeczowski | Grand Master | Polish | 1993-1997 |
| Joseph Frendo Cumbo | Grand Master | Maltese | 1997-2006 |
| Adrian Busietta |
Grand Master Pro Tem |
Maltese | 2006-2006 |
| Nicholas F. S. Papanicolaou | Grand Master |
Greek | 2006- |
